[Effect of shRNA-mediated silence of H-ras gene on proliferation of human SACC-M cells].
Zhonghua Kou Qiang Yi Xue Za Zhi
; 43(2): 113-7, 2008 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-18683737
ABSTRACT
OBJECTIVE:
To examine the effects of H-ras gene silence on cell cycle, proliferation and apoptosis of salivary adenoid cystic carcinoma -M (SACC-M) cell lines.METHODS:
The plasmid H-ras-shRNA, containing the sequence of shRNA targeting H-ras, and HK-shRNA (without interfering effect) were constructed and transfected into SACC-M cells. The cell line with shRNA plasmid stable expression was isolated by G418. The expression levels of H-ras were detected by RT-PCR and protein immunofluorescent assay; cell cycle and cell apoptosis were analyzed by flow cytometry (FCM). The proliferation of cell was also determined by subcutaneous tumor formation in nude mice.RESULTS:
After transfection of H-ras-shRNA plasmid, the mRNA expression of H-ras in SACC-M cells was down-regulated by 61.80% and protein expression of H-ras was inhibited by 62.76%; the cell proliferation was inhibited obviously; the G0G1 phase cells were increased. The cell apoptosis rate of H-ras-shRNA group was significantly higher than that of HK-shRNA group (P <0.05). The volume of subcutaneous tumor in nude mice was significantly smaller in Hras-shRNA group than in control group.CONCLUSIONS:
The recombinant plasmid HRAS-shRNA could efficiently down-regulate the expression of H-ras gene and protein, induce apoptosis of SACC-M cells and simultaneously inhibit proliferation of these cells in vitro and in vivo.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias de las Glándulas Salivales
/
Proteínas Proto-Oncogénicas p21(ras)
/
Carcinoma Adenoide Quístico
/
Silenciador del Gen
/
ARN Interferente Pequeño
Límite:
Animals
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Female
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Humans
Idioma:
Zh
Revista:
Zhonghua Kou Qiang Yi Xue Za Zhi
Asunto de la revista:
ODONTOLOGIA
Año:
2008
Tipo del documento:
Article
País de afiliación:
China