Molecular scintigraphic imaging using 99mTc-transferrin is useful for early detection of synovial inflammation of collagen-induced arthritis mouse.
Rheumatol Int
; 29(2): 153-7, 2008 Dec.
Article
en En
| MEDLINE
| ID: mdl-18696076
ABSTRACT
Transferrin receptor (TfR) is highly expressed on rapidly dividing inflammatory cells, but not in nonproliferating cells. We investigated whether scintigraphic imaging using 99mTechnetium-radiolabeled transferrin (99mTc-Tf) is useful for early detection of synovial inflammation of collagen-induced arthritis (CIA) mouse. 99mTc-Tf conjugate was synthesized to target the TfR in inflamed synovium. 99mTc-Tf scintigraphic images were obtained in nonarthritic normal mouse and advanced phase of CIA mouse. 99mTc-Tf and 99mTc-methylenediphosphonate (99mTc-MDP) bone scintigraphic images were obtained in same early phase of CIA mouse. Western blot analysis, hematoxylin & eosin (H&E), and immunohistochemical staining were performed to determine development of arthritis and expression of TfR. Image analyses revealed that the uptake of 99mTc-Tf in inflamed joints of advanced phase of CIA mouse was markedly higher than those by normal nonarthritic mouse. 99mTc-Tf scintigraphy also showed higher uptake in knee joint prior to significant joint swelling in early phase of CIA mouse but 99mTc-MDP bone scintigraphy does not. These scintigraphic findings are well correlated with the results of Western blot, H&E and immunohistochemical analysis. In conclusion, TfR can be used as a specific target for molecular imaging in CIA mouse, and 99mTc-Tf scintigraphy detects synovial inflammation prior to significant clinical findings in CIA mouse.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Artritis Experimental
/
Sinovitis
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Revista:
Rheumatol Int
Año:
2008
Tipo del documento:
Article