Live neuron morphology automatically reconstructed from multiphoton and confocal imaging data.
J Neurophysiol
; 100(4): 2422-9, 2008 Oct.
Article
en En
| MEDLINE
| ID: mdl-18701753
ABSTRACT
We have developed a fully automated procedure for extracting dendritic morphology from multiple three-dimensional image stacks produced by laser scanning microscopy. By eliminating human intervention, we ensure that the results are objective, quickly generated, and accurate. The software suite accounts for typical experimental conditions by reducing background noise, removing pipette artifacts, and aligning multiple overlapping image stacks. The output morphology is appropriate for simulation in compartmental simulation environments. In this report, we validate the utility of this procedure by comparing its performance on live neurons and test specimens with other fully and semiautomated reconstruction tools.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
/
Microscopía Confocal
/
Neuronas
Límite:
Animals
Idioma:
En
Revista:
J Neurophysiol
Año:
2008
Tipo del documento:
Article
País de afiliación:
Estados Unidos