Screening of Taxol-producing fungi based on PCR amplification from Taxus.
Biotechnol Lett
; 30(12): 2119-23, 2008 Dec.
Article
en En
| MEDLINE
| ID: mdl-18709488
Genes coding for 10-deacetylbaccatin III-10-O-acetyl transferase and C-13 phenylpropanoid side chain-CoA acyltransferase were used as molecular markers for screening of Taxol-producing endophytic fungi. Using PCR, three out of 90 endophytic fungi, isolated from Taxus x media and Taxus yunnanensis, gave positive results. These 3 strains, when grown in 300 ml potato/dextrose liquid medium at 25 degrees C for 10 days, contained 100-160 microg Taxol/g dry wt of mycelium.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paclitaxel
/
Taxus
/
Hongos
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Biotechnol Lett
Año:
2008
Tipo del documento:
Article
Pais de publicación:
Países Bajos