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Supercritical fluid extraction of Beauvericin from maize.
Ambrosino, P; Galvano, F; Fogliano, V; Logrieco, A; Fresa, R; Ritieni, A.
Afiliación
  • Ambrosino P; Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli "Federico II", Parco Gussone, Portici (Napoli) 80055, Italy.
Talanta ; 62(3): 523-30, 2004 Feb 27.
Article en En | MEDLINE | ID: mdl-18969327
Beauvericin (BEA), a supercritical fluid extraction with supercritical carbon dioxide from maize was investigated. Extraction efficiencies under several different extraction conditions were examined. Pressure, temperature, extraction time, organic modifier and water matrix content (10%) were investigated. The best extraction conditions were at a temperature of 60 degrees C, 3200psi, for 30min static extraction time and methanol as modifier solvent. Extraction recovery of 36% without modifier by adding water to the matrix in the extraction vessel (reproducibility relative standard deviations (R.S.D.)=3-5%) were recorded. Extraction recovery of 76.9% with methanol as co-solvent (reproducibility R.S.D.=3-5%) was obtained. Data shows that SFE gives a lower BEA recovery compared to conventional extraction protocol with organic solvents while SFE with modifier and conventional extraction yields are comparable. BEA extract contents were determined by high pressure liquid chromatography (HPLC) with a diode array detector (DAD) at 205nm and BEA peak confirmed by LC-MS. Acetonitrile-water as mobile phase and column C-18 were both tested. Instrumental and analytical parameters were optimized in the range linear interval from 1 to 500mgkg(-1) and reached a detection limit of 2ng.

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Talanta Año: 2004 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Talanta Año: 2004 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Países Bajos