A recombinant, membrane-acting immunotoxin.
Cancer Res
; 51(2): 465-7, 1991 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-1898711
ABSTRACT
The anti-Tac antibody is known to bind to the p55 chain of the human interleukin 2 receptor. An immunotoxin was produced by genetically linking Clostridium perfringens phospholipase C (PLC) to the Fab domain of anti-Tac. For this purpose, the PLC gene, with its own promoter and signal sequence, was fused to the 5' end of the VHCH1 segment of the anti-Tac heavy chain gene. The anti-Tac light chain gene, with an attached bacterial signal sequence, was made part of the same transcriptional unit. Escherichia coli transformed with the construct secreted a recombinant immunotoxin, anti-Tac(Fab)-PLC, in an active form. Anti-Tac(Fab)-PLC bound to cells expressing the interleukin 2 receptor and inhibited protein synthesis, with a 50% inhibitory concentration of 0.02 nM (1.8 ng/ml).
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfolipasas de Tipo C
/
Fragmentos Fab de Inmunoglobulinas
/
Inmunotoxinas
Límite:
Humans
Idioma:
En
Revista:
Cancer Res
Año:
1991
Tipo del documento:
Article