Expression and hormonal regulation of Hoxa10 in canine uterus during the peri-implantation period.
Reprod Domest Anim
; 44(4): 638-42, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-18992082
ABSTRACT
Hoxa10, a homeobox gene, is necessary for endometrial receptivity to blastocyst implantation. The aim of this study was to investigate the differential expression of Hoxa10 in canine uterus during early pregnancy and its regulation under different conditions by in situ hybridization. Hoxa10 mRNA was mainly localized in glandular epithelium and myometrium in canine uterus. There was a low level of Hoxa10 expression in the glandular epithelium on days 6, 12 and 17 of pregnancy. On day 20 of pregnancy when embryo implanted, Hoxa10 mRNA was highly expressed in the glandular epithelium surrounding the embryo, but not in the luminal epithelium. The expression of Hoxa10 mRNA gradually declined from day 23 and reached a low level on day 28. In the myometrium, a low level of Hoxa10 mRNA signal was seen on days 6, 12 and 17 of pregnancy and reached a high level on day 20 of pregnancy. During the estrous cycle, a high level of Hoxa10 mRNA expression was seen in the estrous uterus. Either estrogen or progesterone significantly induced the expression of Hoxa10 mRNA in the ovariectomized canine uterus. These results suggest that Hoxa10 expression is closely related to canine embryo implantation and upregulated by estrogen and progesterone.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Implantación del Embrión
/
Útero
/
Expresión Génica
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Regulación hacia Arriba
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Proteínas de Homeodominio
/
Perros
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Reprod Domest Anim
Asunto de la revista:
MEDICINA REPRODUTIVA
/
MEDICINA VETERINARIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
China