Inotodiol, a lanostane triterpenoid, from Inonotus obliquus inhibits cell proliferation through caspase-3-dependent apoptosis.
Anticancer Res
; 28(5A): 2691-6, 2008.
Article
en En
| MEDLINE
| ID: mdl-19035296
ABSTRACT
BACKGROUND:
To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed. MATERIALS ANDMETHODS:
Cell viability was measured by MTT assay. Caspase-3/7 activity and DNA fragmentation were evaluated to analyze apoptosis induction. The in vivo antitumor effect was evaluated by the number of survival days of mouse leukemia P388-bearing female CDF1 mice.RESULTS:
The chloroform extract of I. obliquus sclerotia inhibited proliferation of the P388 cells. Among the triterpenoids examined, only inotodiol inhibited P388 cell proliferation. DNA fragmentation and caspase-3/7 activation were observed in the P388 cells treated with inotodiol (30 microM). A caspase-3 inhibitor, DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-al, 100 microM) partially inhibited the DNA fragmentation and growth-inhibition induced by inotodiol. The intraperitoneal administration of 10 mg/kg inotodiol prolonged the number of survival days of the P388-bearing mice.CONCLUSION:
Inotodiol inhibits cell proliferation through apoptosis induction by activating caspase-3.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Leucemia P388
/
Apoptosis
/
Caspasa 3
/
Lanosterol
Límite:
Animals
Idioma:
En
Revista:
Anticancer Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón