Determination of N-ethylglycine in urine of cancer patients with metastatic bone disease by HPLC using 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride as a fluorescent labeling reagent.
Anal Sci
; 24(12): 1629-31, 2008 Dec.
Article
en En
| MEDLINE
| ID: mdl-19075476
ABSTRACT
A simple and highly sensitive HPLC for the determination of N-ethylglycine in urine was developed. The labeling reaction of N-ethylglycine with 4-(5,6-dimethoxy-2-phthalimidinyl)-2-methoxyphenylsulfonyl chloride was carried out at 70 degrees C for 15 min at pH 9.0. The fluorescent derivative was separated on a reversed-phase column and detected at excitation and emission wavelengths of 320 and 400 nm, respectively. The detection limit of N-ethylglycine was 15 fmol (S/N = 3). The recovery of N-ethylglycine added to urine was 101.9%. The concentration of N-ethylglycine in urine of cancer patients with metastatic bone disease was 11.3 +/- 22.0 nmol/mg creatinine, and that of normal subject was 0.4 +/- 0.4 nmol/mg creatinine.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ftalimidas
/
Neoplasias Óseas
/
Glicinas N-Sustituídas
/
Colorantes Fluorescentes
Tipo de estudio:
Diagnostic_studies
/
Observational_studies
Límite:
Adult
/
Humans
/
Middle aged
Idioma:
En
Revista:
Anal Sci
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón