Isolation, expansion, and differentiation of mouse skin-derived precursors.
Methods Mol Biol
; 482: 159-70, 2009.
Article
en En
| MEDLINE
| ID: mdl-19089355
ABSTRACT
The isolation and experimental manipulation of multipotent precursors is of increasing therapeutic relevance. We recently reported the generation of cultures of Skin-derived Precursors ('SKPs'), multipotent cells that can be isolated from the dermis of embryonic, neonatal, and adult rodent skin (1), and from adult human skin (2) SKPs have similarities to stem cells of the embryonic neural crest (3), and differentiate into a variety of neural and mesodermal cell phenotypes, including peripheral neurons and glial cells, smooth muscle cells, bone, cartilage, and adipocytes (3-5). Here, we detail the establishment, propagation, neural differentiation, and immunocytochemical analysis of SKP cultures.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Piel
/
Células Madre
/
Diferenciación Celular
/
Separación Celular
Límite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2009
Tipo del documento:
Article
País de afiliación:
Canadá