Oropouche fever epidemic in Northern Brazil: epidemiology and molecular characterization of isolates.
J Clin Virol
; 44(2): 129-33, 2009 Feb.
Article
en En
| MEDLINE
| ID: mdl-19117799
ABSTRACT
BACKGROUND:
Oropouche fever virus is an important arbovirus associated with febrile disease that re-emerged in 2006 in several municipalities of Pará State, Bragantina region, Amazon, Brazil, 26 years after the last epidemic.OBJECTIVE:
To investigate an Oropouche fever outbreak in this region. STUDYDESIGN:
A serologic survey and prospective study of acute febrile cases were performed in Magalhães Barata (urban and rural areas) and Maracanã (rural area) municipalities. Serology (IgM-ELISA and hemagglutination-inhibition [HI]), virus isolation, RT-PCR and real-time-PCR were used to confirm Oropouche virus (OROV) as responsible for the febrile outbreaks.RESULTS:
Real-time-PCR showed high titers of OROV in acute-phase serum samples from febrile patients. From 113 of 119 acutely febrile patients with paired serum samples, OROV infections was confirmed by serologic conversion (n=76) or high titers (n=37) for both HI and IgM-ELISA. Patients had a febrile disease characterized by headache, chills, dizziness, photophobia, myalgia, nausea, and vomiting. Females and children under 15 years of age were most affected. Nucleotide sequencing of six OROV isolates identified that genotype II was associated with the human disease epidemic.CONCLUSIONS:
Oropouche fever, which has re-emerged in the Bragantina region in eastern Amazon 26 years after the last epidemic, is caused by genotype II, a lineage previously found only in Peru and western Brazil.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Brotes de Enfermedades
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Orthobunyavirus
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Infecciones por Bunyaviridae
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Anticuerpos Antivirales
Tipo de estudio:
Etiology_studies
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Observational_studies
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Prognostic_studies
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Screening_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Aged80
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Child
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Child, preschool
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Female
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Humans
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Infant
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Male
País/Región como asunto:
America do sul
/
Brasil
Idioma:
En
Revista:
J Clin Virol
Asunto de la revista:
VIROLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Brasil