Self-etching increases matrix metalloproteinase expression in the dentin-pulp complex.
J Dent Res
; 88(1): 77-82, 2009 Jan.
Article
en En
| MEDLINE
| ID: mdl-19131322
ABSTRACT
In adhesive restorations, one major problem is hybrid layer degradation. At present, this deterioration is explained by the activation of the endogenous matrix metalloproteinases (MMPs) present in dentin due to the acidic property of adhesive systems. We hypothesized that self-etching adhesive should also stimulate the expression of MMPs in odontoblasts. In cultured tooth slices, we evaluated the changes in MMP-2 and proMMP-9 expression in the dentin-pulp complex after self-etching adhesive treatment on dentin cavities in immunochemistry and by zymography. The treatment resulted in increased MMP-2 expression in odontoblasts, as shown by immunohistochemistry. Zymography showed increased proMMP-9 and MMP-2 in dentin under self-etching treatment when pulp was present. These results showed that self-etching adhesive stimulates the secretion of MMPs from the dentin-pulp complex and, more precisely, by odontoblasts, suggesting that odontoblasts participate in hybrid layer degradation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recubrimientos Dentinarios
/
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 9 de la Matriz
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Pulpa Dental
/
Dentina
Tipo de estudio:
Clinical_trials
Límite:
Adolescent
/
Humans
Idioma:
En
Revista:
J Dent Res
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA