Supercoil-accelerated DNA threading intercalation.
Biochemistry
; 48(7): 1442-4, 2009 Feb 24.
Article
en En
| MEDLINE
| ID: mdl-19161310
ABSTRACT
The effect of DNA supercoiling on a sterically very demanding threading intercalation process is investigated here. We find that the threading rate of a dimeric ruthenium complex into a negatively supercoiled plasmid at low binding density is 2 orders of magnitude higher than into the cleaved linear form. Further saturation is on the other hand kinetically hampered in comparison to the relaxed DNA. We also observe that threading kinetics correlates with the inhibition of luciferase expression from the plasmid construct. The results show how the target torsional strain can function as a control of DNA threading kinetics and gene expression efficiency.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Superhelicoidal
/
Sustancias Intercalantes
Idioma:
En
Revista:
Biochemistry
Año:
2009
Tipo del documento:
Article
País de afiliación:
Suecia