Expression, purification, crystallization and preliminary X-ray studies of the Ebola VP35 interferon inhibitory domain.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 65(Pt 2): 163-5, 2009 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-19194011
ABSTRACT
Ebola VP35 is a multifunctional protein that is important for host immune suppression and pathogenesis. VP35 contains an N-terminal oligomerization domain and a C-terminal interferon inhibitory domain (IID). Mutations within the VP35 IID result in loss of host immune suppression. Here, efforts to crystallize recombinantly overexpressed VP35 IID that was purified from Escherichia coli are described. Native and selenomethionine-labeled crystals belonging to the orthorhombic space group P2(1)2(1)2(1) were obtained by the hanging-drop vapor-diffusion method and diffraction data were collected at the ALS synchrotron.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Difracción de Rayos X
/
Regulación Viral de la Expresión Génica
/
Proteínas del Núcleo Viral
/
Interferones
/
Ebolavirus
/
Nucleoproteínas
Idioma:
En
Revista:
Acta Crystallogr Sect F Struct Biol Cryst Commun
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos