Aberration-corrected microscopy for structural biology applications.
J Microsc
; 233(1): 170-7, 2009 Jan.
Article
en En
| MEDLINE
| ID: mdl-19196423
ABSTRACT
This study explores the potential of a C(s)-corrected transmission electron microscope for structural studies of biological samples, in particular isolated macromolecular complexes. A 300-kV transmission electron microscope, equipped with a C(s) corrector was employed to record sets of images at different defocus and C(s) settings. The experiments were designed to determine whether imaging with large defocus benefits from C(s) correction. Defocus contrast in biological imaging has a stronger influence on image resolution than any other parameter. We find the results are in good agreement with theoretical framework, verifying that the typical imaging conditions required for biological investigations are not affected by C(s) correction.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Procesamiento de Imagen Asistido por Computador
/
Microscopía Electrónica de Transmisión
Idioma:
En
Revista:
J Microsc
Año:
2009
Tipo del documento:
Article
País de afiliación:
Alemania