Assaying of autophagic protein degradation.
Methods Enzymol
; 452: 47-61, 2009.
Article
en En
| MEDLINE
| ID: mdl-19200875
ABSTRACT
Macroautophagy is a three-step process (1) autophagosomes form and mature, (2) the autophagosomes fuse with lysosomes, and (3) the autophagic cargo is degraded in the lysosomes. It is this lysosomal degradation of the autophagic cargo that constitutes the autophagic flux. As in the case of metabolic pathways, the steady-state concentration of the intermediary autophagic structures alone is insufficient for investigating the flux. Assaying the degradation of long-lived proteins as described in this chapter is one of the methods that can be used to measure autophagic flux.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Autofagia
/
Bioensayo
/
Proteínas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Enzymol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia