Secretory expression of K88 (F4) fimbrial adhesin FaeG by recombinant Lactococcus lactis for oral vaccination and its protective immune response in mice.
Biotechnol Lett
; 31(7): 991-7, 2009 Jul.
Article
en En
| MEDLINE
| ID: mdl-19277476
ABSTRACT
K88 (F4) fimbrial adhesin, FaeG, was expressed extracellularly in Lactococcus lactis using a nisin-controlled gene expression system. The antibody response and protective efficacy of the recombinant bacteria (L. lactis [spNZ8048-faeG]) against live enterotoxigenic E. coli (ETEC) C(83549) challenge were evaluated in ICR mice. Mice vaccinated with L. lactis [spNZ8048-faeG] had a significantly increased antigen-specific IgG level in the serum and decreased mortality rate (P < 0.05) compared with the control. This indicates that oral immunization of L. lactis [spNZ8048-faeG] can induce an immune-response protection upon challenge with live ETEC in ICR mice.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Lactococcus lactis
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Adhesinas de Escherichia coli
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Vacunas contra Escherichia coli
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Infecciones por Escherichia coli
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Escherichia coli Enterotoxigénica
Límite:
Animals
Idioma:
En
Revista:
Biotechnol Lett
Año:
2009
Tipo del documento:
Article