Gene transfer into mammalian cells using a self-deleting avian leukemia and sarcoma virus-based vector.
Intervirology
; 51(6): 447-52, 2008.
Article
en En
| MEDLINE
| ID: mdl-19321931
ABSTRACT
OBJECTIVES:
We have previously described an avian leukemia and sarcoma virus-based vector containing an additional att sequence in an internal position that is capable of self-deleting most of its 5' viral sequences during one cycle of replication in avian cells [Virus Res 2008;13572-82; Arch Virol 2008;1532233-2243]. Herein, our aim was to test the infectivity and self-deleting properties of this avian retroviral vector in human cells.METHODS:
Human Hela cells transiently expressing the cellular receptor for avian leukemia and sarcoma viruses (tva) were infected with the avian vector. Molecular analyses of thirteen clones were performed.RESULTS:
Data showed that more than 77% of proviruses had lost the 5' part of their genome including the selectable gene. At least 61% of these proviruses were flanked on the left by the additional att sequence and on the right by the LTR. None of the thirteen proviruses was able to express a full-length genomic RNA.CONCLUSION:
This study demonstrates that the self-deleting properties of the avian vector in avian cells may be also applicable to human cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Eliminación de Secuencia
/
Técnicas de Transferencia de Gen
/
Alpharetrovirus
/
Vectores Genéticos
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Intervirology
Año:
2008
Tipo del documento:
Article
País de afiliación:
Francia