Antibody responses in the lungs of mice following oral immunization with Salmonella typhimurium aroA and invasive Escherichia coli strains expressing the filamentous hemagglutinin of Bordetella pertussis.
Infect Immun
; 59(12): 4391-7, 1991 Dec.
Article
en En
| MEDLINE
| ID: mdl-1937797
ABSTRACT
The filamentous hemagglutinin (FHA) of Bordetella pertussis was expressed in the attenuated aroA mutant of Salmonella typhimurium, SL3261, and in a strain of Escherichia coli harboring Shigella flexneri plasmid pWR110, which encodes bacterial invasiveness for epithelial cells. Expression of FHA in these strains did not interfere with their ability to invade Henle cells. Immunoglobulins A and G specific for FHA were detected in lung washes of mice following oral immunization with the live recombinant organisms; antibody levels were significantly higher than those in mice immunized with killed bacteria administered orally or intraperitoneally. Live oral vaccines carrying protective antigens of B. pertussis may be an important alternative to new-generation component vaccines against whooping cough.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Salmonella typhimurium
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Bordetella pertussis
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Factores de Virulencia de Bordetella
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Vacunas Bacterianas
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Vacunas Sintéticas
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Adhesinas Bacterianas
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Escherichia coli
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Hemaglutininas
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Pulmón
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Anticuerpos Antibacterianos
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Infect Immun
Año:
1991
Tipo del documento:
Article
País de afiliación:
Alemania