Phenotype profiling of single gene deletion mutants of E. coli using Biolog technology.
Genome Inform
; 21: 42-52, 2008.
Article
en En
| MEDLINE
| ID: mdl-19425146
ABSTRACT
Phenotype MicroArray (PM) technology is high-throughput phenotyping system and is directly applicable to assay the effects of genetic changes in cells. In this study, we performed comprehensive PM analysis using single gene deletion mutants of central metabolic pathway and related genes. To elucidate the structure of central metabolic networks in Escherichia coli K-12, we focused 288 different PM conditions of carbon and nitrogen sources and performed bioinformatic analysis. For data processing, we employed noise reduction procedures. The distance between each of the mutants was defined by Manhattan distance and agglomerative Ward's hierarchical method was applied for clustering analysis. As a result, five clusters were revealed which represented to activate or repress cellular respiratory activities. Furthermore, the results might suggest that Glyceraldehyde-3P plays a key role as a molecular switch of central metabolic network.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Eliminación de Gen
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Perfilación de la Expresión Génica
/
Escherichia coli K12
/
Mutación
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Genome Inform
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón