Digital in-line holography with femtosecond VUV radiation provided by the free-electron laser FLASH.
Opt Express
; 17(10): 8220-8, 2009 May 11.
Article
en En
| MEDLINE
| ID: mdl-19434154
ABSTRACT
Femtosecond vacuum ultraviolet (VUV) radiation provided by the free-electron laser FLASH was used for digital in-line holographic microscopy and applied to image particles, diatoms and critical point dried fibroblast cells. To realize the classical in-line Gabor geometry, a 1 microm pinhole was used as spatial filter to generate a divergent light cone with excellent pointing stability. At a fundamental wavelength of 8 nm test objects such as particles and diatoms were imaged at a spatial resolution of 620 nm. In order to demonstrate the applicability to biologically relevant systems, critical point dried rat embryonic fibroblast cells were for the first time imaged with free-electron laser radiation.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Rayos Ultravioleta
/
Holografía
/
Electrones
/
Rayos Láser
Límite:
Animals
Idioma:
En
Revista:
Opt Express
Asunto de la revista:
OFTALMOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Alemania