Organic solvent extraction and metabonomic profiling of the metabolites in erythrocytes.
J Chromatogr B Analyt Technol Biomed Life Sci
; 877(18-19): 1751-7, 2009 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-19467933
ABSTRACT
We used erythrocytes as the model tissue to evaluate an optimal solution for the extraction of intracellular metabolites and time-dependent variation of the metabolome in living cells. Projection to latent structure (PLS) of the GC/MS and LC/MS data suggested that the most efficient solution for the extraction of metabolites from wet erythrocytes (50 mg) could be a methanol-chloroform-water mixture (950 microL, 70020050, v/v/v). PLS-discriminant analysis (DA) clearly profiled a time-dependent alternation of metabolic phenotype of erythrocytes. Identification of the metabolites showed that the process was characterized by accumulating of metabolic products and depleting of nutritious substances in erythrocytes during incubation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Solventes
/
Eritrocitos
/
Metabolómica
/
Cromatografía de Gases y Espectrometría de Masas
Límite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
China