Evidence that ecotropic murine leukemia virus contamination in TZM-bl cells does not affect the outcome of neutralizing antibody assays with human immunodeficiency virus type 1.
J Virol
; 83(16): 8289-92, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-19474095
ABSTRACT
The TZM-bl cell line that is commonly used to assess neutralizing antibodies against human immunodeficiency virus type 1 (HIV-1) was recently reported to be contaminated with an ecotropic murine leukemia virus (MLV) (Y. Takeuchi, M. O. McClure, and M. Pizzato, J. Virol. 8212585-12588, 2008), raising questions about the validity of results obtained with this cell line. Here we confirm this observation and show that HIV-1 neutralization assays performed with a variety of serologic reagents in a similar cell line that does not harbor MLV yield results that are equivalent to those obtained in TZM-bl cells. We conclude that MLV contamination has no measurable effect on HIV-1 neutralization when TZM-bl cells are used as targets for infection.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pruebas de Neutralización
/
Infecciones por VIH
/
Línea Celular
/
VIH-1
/
Virus de la Leucemia Murina
Límite:
Humans
Idioma:
En
Revista:
J Virol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos