IRAP identifies an endosomal compartment required for MHC class I cross-presentation.
Science
; 325(5937): 213-7, 2009 Jul 10.
Article
en En
| MEDLINE
| ID: mdl-19498108
ABSTRACT
Major histocompatibility complex (MHC) class I molecules present peptides, produced through cytosolic proteasomal degradation of cellular proteins, to cytotoxic T lymphocytes. In dendritic cells, the peptides can also be derived from internalized antigens through a process known as cross-presentation. The cellular compartments involved in cross-presentation remain poorly defined. We found a role for peptide trimming by insulin-regulated aminopeptidase (IRAP) in cross-presentation. In human dendritic cells, IRAP was localized to a Rab14+ endosomal storage compartment in which it interacted with MHC class I molecules. IRAP deficiency compromised cross-presentation in vitro and in vivo but did not affect endogenous presentation. We propose the existence of two pathways for proteasome-dependent cross-presentation in which final peptide trimming involves IRAP in endosomes and involves the related aminopeptidases in the endoplasmic reticulum.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cistinil Aminopeptidasa
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Endosomas
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Células Dendríticas
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Antígenos de Histocompatibilidad Clase I
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Presentación de Antígeno
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Reactividad Cruzada
Límite:
Animals
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Humans
Idioma:
En
Revista:
Science
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia
Pais de publicación:
EEUU
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ESTADOS UNIDOS
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ESTADOS UNIDOS DA AMERICA
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EUA
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UNITED STATES
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UNITED STATES OF AMERICA
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US
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USA