Down-regulated miR-9 and miR-433 in human gastric carcinoma.
J Exp Clin Cancer Res
; 28: 82, 2009 Jun 16.
Article
en En
| MEDLINE
| ID: mdl-19531230
ABSTRACT
BACKGROUND:
MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.METHODS:
To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.RESULTS:
We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.CONCLUSION:
Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Neoplasias Gástricas
/
ARN Neoplásico
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Regulación hacia Abajo
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Regulación Neoplásica de la Expresión Génica
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MicroARNs
Límite:
Humans
Idioma:
En
Revista:
J Exp Clin Cancer Res
Año:
2009
Tipo del documento:
Article