Molecular cloning of three cDNAs that encode cysteine proteinases in the digestive gland of the American lobster (Homarus americanus).
FEBS Lett
; 292(1-2): 115-20, 1991 Nov 04.
Article
en En
| MEDLINE
| ID: mdl-1959590
ABSTRACT
Three clones were isolated from a lobster digestive gland cDNA library, using oligonucleotide probes based on the partial amino terminal sequence of a digestive cysteine proteinase. The cDNAs, LCP1, LCP2 and LCP3 encode preproenzymes of 322, 323 and 321 amino acid residues, and putative mature enzymes of 217, 216 and 215 residues, respectively. Calculated mature protein molecular masses are 23386 (LCP1), 29093 (LCP2) and 23255 (LCP3) Sequence alignments show that the lobster enzymes are more similar to L (55-62% identity) than H (42-44%) or B (22-24%) cathepsins. Southern analysis indicated as many as eleven genes related to the three cDNAs.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Cisteína Endopeptidasas
/
Sistema Digestivo
/
Nephropidae
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1991
Tipo del documento:
Article