[An update on antifungal susceptibility testing]. / Actualización en pruebas de susceptibilidad antifúngica.
Rev Chilena Infectol
; 26(2): 144-50, 2009 Apr.
Article
en Es
| MEDLINE
| ID: mdl-19621145
Due to increasing of invasive fungal infections and emergeney of antifungal drugs resistant fungi, standardized methods of antifungal susceptibility testing (AST) have been developed. The Clinical Laboratory Standards Instutute (CLSI) and the European for Committee Antimicrobial Susceptibility Testing (EUCAST) have guidelines for susceptibility of yeasts by broth microdilution (M27-A2 and E. Dis. 7.1 documents, respectively). Both are equivalent, although they present methodological and interpretative breakpoints differences. In addition, the CLSI have the M38-A (for filamentous fungi) and M44-A (disk diffusion) documents, whereas EUCAST is developing a document for Aspergillus spp. Furthermore, commercial methods are available that display good correlation with the methods of reference such as E-test, Sensititre and Vitek2. The interpretation of the results must be careful because the determination of the minimum inhibitory concentration (CIM) is difficult for fungi, there are host factors involved and not always there is a correlation between MIC and clinical outcome. Due to these methods are laborious and require trained personnel, to ask for AST to a reference laboratory is recommendable.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pruebas de Sensibilidad Microbiana
/
Hongos
/
Antifúngicos
Tipo de estudio:
Guideline
Idioma:
Es
Revista:
Rev Chilena Infectol
Año:
2009
Tipo del documento:
Article
País de afiliación:
Chile
Pais de publicación:
Chile