[Study on expression of curcin gene cloned from Jatropha curcas in Escherichia coli].
Zhongguo Zhong Yao Za Zhi
; 34(6): 656-9, 2009 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-19623998
ABSTRACT
OBJECTIVE:
To construct mature protein curcin gene prokaryotic expression vectors in Escherichia coli and choose the optimal inducing condition of the recombinant strains.METHOD:
The gene encoding of curcin was amplified from the genome of Jatropha curcas seeds by PCR and cloned into the expression vectors pQE-30 and pET-32 obtaining recombinant vectors pQE-R and pET-R respectively. The two vectors were transferred into E. coli BL21 (DE3) and the recombinant strains PRM and PRB were attained respectively. PRM and PRB were induced by different revulsants and under different temperature and time.RESULT:
The gene encoding of mature protein curcin was amplified by PCR and the recombinant strains PRM and PRB were obtained.CONCLUSION:
The results showed that PRB could not produce recombinant protein under such conditions. However, PRM could highly produce recombinant protein induced by 0.5 mmol x L(-1) IPTG at 28 degrees C for 6 h.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Jatropha
/
Escherichia coli
/
Proteínas Inactivadoras de Ribosomas Tipo 1
Idioma:
Zh
Revista:
Zhongguo Zhong Yao Za Zhi
Asunto de la revista:
FARMACOLOGIA
/
TERAPIAS COMPLEMENTARES
Año:
2009
Tipo del documento:
Article
País de afiliación:
China