Aptamer nano-flares for molecular detection in living cells.
Nano Lett
; 9(9): 3258-61, 2009 Sep.
Article
en En
| MEDLINE
| ID: mdl-19645478
ABSTRACT
We demonstrate a composite nanomaterial, termed an aptamer nano-flare, that can directly quantify an intracellular analyte in a living cell. Aptamer nano-flares consist of a gold nanoparticle core functionalized with a dense monolayer of nucleic acid aptamers with a high affinity for adenosine triphosphate (ATP). The probes bind selectively to target molecules and release fluorescent reporters which indicate the presence of the analyte. Additionally, these nanoconjugates are readily taken up by cells where their signal intensity can be used to quantify intracellular analyte concentration. These nanoconjugates are a promising approach for the intracellular quantification of other small molecules or proteins, or as agents that use aptamer binding to elicit a biological response in living systems.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adenosina Trifosfato
/
Aptámeros de Nucleótidos
/
Nanopartículas del Metal
/
Oro
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Nano Lett
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos