[Mechanism related to inhibition of leukemia K562 cells by berbamine].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 38(4): 387-91, 2009 Jul.
Article
en Zh
| MEDLINE
| ID: mdl-19693977
ABSTRACT
OBJECTIVE:
To investigate the mechanism related to the inhibition of leukemia K562 cells by berbamine.METHODS:
After K562 cells were treated with 8 microg/ml berbamine, the expression levels of NF-kappaB, IkappaBalpha, pIkappaBalpha, IKKalpha, A20 were determined by Western blot.RESULTS:
With the exposure time extending, the total NF-kappaB showed no significant changes,but NF-kappaB expression in nucleus was decreased dramatically after treated with berbamine for 24 h. The ratio of nucleus NF-kappaB/histone H1 was decreased from 59.2%,gradually to 31.4%,19.7%,4.1%,and 0%. At the same time,the expression of A20 was increased,while the expression of pIkappaBalpha, IKKalpha was down-regulated.CONCLUSION:
Berbamine may induce K562 cell apoptosis through NF-kappaB pathway. Down-regulation of NF-kappaB and bcr-abl gene expression might be involved in cell apoptosis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Regulación hacia Abajo
/
FN-kappa B
/
Apoptosis
/
Bencilisoquinolinas
/
Antineoplásicos Fitogénicos
Límite:
Humans
Idioma:
Zh
Revista:
Zhejiang Da Xue Xue Bao Yi Xue Ban
Asunto de la revista:
MEDICINA
Año:
2009
Tipo del documento:
Article
País de afiliación:
China