Evaluation of mobility of 2-N-tert-butylaminoxyladenosine incorporated into oligodeoxynucleotides.
Nucleic Acids Symp Ser (Oxf)
; (53): 151-2, 2009.
Article
en En
| MEDLINE
| ID: mdl-19749305
ABSTRACT
We synthesized oligodeoxynucleotide (ODN, 3) containing 2-N-tert-butylaminoxyladenosine (1) and studied EPR spectra of 3 and its duplexes. The h(+)/h(0) values in the EPR spectra of duplexes between 3 and 5-8 well correlated with Tm values. We also synthesized ODN 4 containing 2, which has a cyclic aminoxyl via a linker, to compare with ODN 3. The h(+)/h(0) values in the EPR spectra of duplexes between 4 and 5-8 did not correlate with Tm values. These results indicate that 1 has a potential to monitor of motion of the nucleobase.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oligodesoxirribonucleótidos
/
Marcadores de Spin
/
Adenosina
Idioma:
En
Revista:
Nucleic Acids Symp Ser (Oxf)
Año:
2009
Tipo del documento:
Article
País de afiliación:
Japón