Comparison of enrichment procedures for shiga toxin-producing Escherichia coli in wastes from commercial swine farms.
J Food Prot
; 72(9): 1982-6, 2009 Sep.
Article
en En
| MEDLINE
| ID: mdl-19777903
ABSTRACT
Three methods for enrichment of Shiga toxin-producing Escherichia coli (STEC) were compared using waste pit samples from swine production facilities housing 50 to 3,000 animals. The STEC gene stx2 was detected in 5 of 17 pooled samples using a U.S. Department of Agriculture (USDA) enrichment procedure, 6 of 17 samples using a U.S. Food and Drug Administration (FDA) enrichment procedure, and 8 of 17 samples using an experimental acid enrichment. All isolates were non-O157 and 5 of 6 were positive for enterotoxigenic E. coli-associated heat stable toxins a and b. The three enrichment procedures were also tested for their ability to support growth of 31 strains of STEC. The acid enrichment media supported growth of 100% of the strains, the FDA medium supported 77% of the strains, and the USDA medium supported 16% of the strains.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Recuento de Colonia Microbiana
/
Medios de Cultivo
/
Escherichia coli Shiga-Toxigénica
/
Heces
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Food Prot
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos