[Rapid detection and identification of mycobacterial DNA by PCR].
Rinsho Byori
; 38(11): 1247-53, 1990 Nov.
Article
en Ja
| MEDLINE
| ID: mdl-1980318
ABSTRACT
We have developed a highly sensitive and rapid PCR assay for detection and identification of mycobacterial species. Two sets of PCR primers were prepared; one was for the 19 kDa antigen gene and the other for the dna J gene. The PCR for 19 kDa antigen gene was found to be specific to the M. tuberculosis complex; M. tuberculosis, M. bovis and M. africanum. On the other hand, the PCR for the dna J gene was able to detect a broad spectrum of mycobacterial species including M. tuberculosis, M. avium, M. intracellulare and M. kansasii. The sensitivity of PCR was similar to that of the culture method for precultured M. tuberculosis whereas PCR was much more sensitive than the culture for clinical samples. The procedure of decontamination by sodium hydroxide for clinical samples could reduce the viability of M. tuberculosis while it did not affect mycobacterial DNA. The nucleotide sequence homologies among major mycobacteria were also determined following PCR for the dna J gene. This allowed us to make restriction maps for each mycobacterium. We have demonstrated that the PCR-RFLP for the dna J gene will be a useful laboratory test for rapid identification of tuberculous and nontuberculous mycobacterial species.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
Genes Bacterianos
/
Mycobacterium
Tipo de estudio:
Diagnostic_studies
Idioma:
Ja
Revista:
Rinsho Byori
Año:
1990
Tipo del documento:
Article