Activation of sigma 28-dependent transcription in Escherichia coli by the cyclic AMP receptor protein requires an unusual promoter organization.
Mol Microbiol
; 75(5): 1098-111, 2010 Mar.
Article
en En
| MEDLINE
| ID: mdl-19843224
ABSTRACT
The Escherichia coli aer regulatory region contains a single promoter that is recognized by RNA polymerase containing the flagellar sigma factor, sigma(28). Expression from this promoter is dependent on direct activation by the cyclic AMP receptor protein, which binds to a target centred 49.5 base pairs upstream from the transcript start. Activator-dependent transcription from the aer promoter was reconstituted in vitro, and a tethered inorganic nuclease was used to find the position of the C-terminal domains of the RNA polymerase alpha subunits in transcriptionally competent open complexes. We report that the ternary activator--RNA polymerase--aer promoter open complex is organized differently from complexes at previously characterized promoters. Among other E. coli promoters recognized by RNA polymerase containing sigma(28), only the trg promoter is activated directly by the cyclic AMP receptor protein. The organization of the different promoter elements and the activator binding site at the trg promoter is the same as at the aer promoter, suggesting a common activation mechanism.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factor sigma
/
Proteínas Bacterianas
/
Transcripción Genética
/
Proteínas Portadoras
/
Receptores de AMP Cíclico
/
Regiones Promotoras Genéticas
/
Proteínas de Escherichia coli
/
Proteínas de Unión al ADN
/
Escherichia coli
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Mol Microbiol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
MICROBIOLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Reino Unido