Pathogenesis of aerosolized Eastern Equine Encephalitis virus infection in guinea pigs.
Virol J
; 6: 170, 2009 Oct 23.
Article
en En
| MEDLINE
| ID: mdl-19852817
ABSTRACT
Mice and guinea pigs were experimentally exposed to aerosols containing regionally-distinct strains (NJ1959 or ArgM) of eastern equine encephalitis virus (EEEV) at two exclusive particle size distributions. Mice were more susceptible to either strain of aerosolized EEEV than were guinea pigs; however, clinical signs indicating encephalitis were more readily observed in the guinea pigs. Lower lethality was observed in both species when EEEV was presented at the larger aerosol distribution (> 6 mum), although the differences in the median lethal dose (LD50) were not significant. Virus isolation and immunohistochemistry indicated that virus invaded the brains of guinea pigs within one day postexposure, regardless of viral strain or particle size distribution. Immunohistochemistry further demonstrated that neuroinvasion occurred through the olfactory system, followed by transneuronal spread to all regions of the brain. Olfactory bipolar neurons and neurons throughout the brain were the key viral targets. The main microscopic lesions in infected guinea pigs were neuronal necrosis, inflammation of the meninges and neuropil of the brain, and vasculitis in the brain. These results indicate that guinea pigs experimentally infected by aerosolized EEEV recapitulate several key features of fatal human infection and thus should serve as a suitable animal model for aerosol exposure to EEEV.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aerosoles
/
Virus de la Encefalitis Equina del Este
/
Encefalomielitis Equina
Tipo de estudio:
Etiology_studies
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Virol J
Asunto de la revista:
VIROLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos