Platelet adhesion and aggregation under flow using microfluidic flow cells.
J Vis Exp
; (32)2009 Oct 27.
Article
en En
| MEDLINE
| ID: mdl-19859055
ABSTRACT
Platelet aggregation occurs in response to vascular injury where the extracellular matrix below the endothelium has been exposed. The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions. Extracellular proteins, collagen I or von Willebrand factor are deposited within the microfluidic channel using active perfusion with a pneumatic pump. The matrix proteins are then washed with buffer and blocked to prepare the microfluidic channel for platelet interactions. Whole blood labeled with fluorescent dye is perfused through the channel at various flow rates in order to achieve platelet activation and aggregation. Inhibitors of platelet aggregation can be added prior to the flow cell experiment to generate IC50 dose response data.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Adhesividad Plaquetaria
/
Agregación Plaquetaria
/
Técnicas Analíticas Microfluídicas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Vis Exp
Año:
2009
Tipo del documento:
Article