Differential effects of nonylphenol on testosterone secretion in rat Leydig cells.
Toxicology
; 268(1-2): 1-7, 2010 Jan 31.
Article
en En
| MEDLINE
| ID: mdl-19883722
ABSTRACT
Nonylphenol (NP), a final metabolite of nonylphenol polyethoxylate, has been reported to interfere with male reproduction. However, its mechanisms are not fully understood. In the present study, we examined the effects of NP on steroidogenesis of testosterone in rat Leydig cells. The testosterone concentrations in rat plasma were examined after intravenous injection of NP (100 microg/kg) at different time intervals. In addition, rat Leydig cells were challenged with different concentrations of NP (4.25-127.5 microM) to evaluate its influences on testosterone steroidogenesis. Administration of NP showed a decrease of hCG-induced plasma testosterone. Moreover, in vitro experiments revealed that NP (127.5 microM) alone stimulated testosterone release through increase of both protein levels and activities of the StAR and P450(SCC). In contrast, NP inhibited hCG-induced testosterone release in rat Leydig cells. The inhibitory effect was also observed after incubation of the Leydig cells in the presence of different precursors. These results suggested that NP had differential effects on testosterone synthesis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fenoles
/
Testosterona
/
Células Intersticiales del Testículo
Límite:
Animals
Idioma:
En
Revista:
Toxicology
Año:
2010
Tipo del documento:
Article