Protein fixation and antigen retrieval: chemical studies.
Biotech Histochem
; 84(5): 217-21, 2009 Oct.
Article
en En
| MEDLINE
| ID: mdl-19886758
ABSTRACT
Abstract Fixation with formaldehyde is the first process to which most biopsy and necropsy specimens are exposed prior to dehydration and embedding in paraffin wax. Tissue specimens that have been fixed in formaldehyde have architectural characteristics that are familiar to virtually every pathologist and these facilitate routine diagnosis. Nevertheless, formaldehyde fixation has some deleterious effects including reduction in immunoreactivity and degradation of nucleic acids. Development of methods to counteract these deleterious effects requires an understanding of the chemical events that occur during tissue fixation and subsequent tissue processing. This short review illustrates some of the chemical consequences of formaldehyde fixation and ethanol dehydration. It also provides some insight into the molecular events accompanying heat-induced antigen retrieval.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Epítopos
Límite:
Humans
Idioma:
En
Revista:
Biotech Histochem
Asunto de la revista:
HISTOCITOQUIMICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos