Antibodies to endogenous tear protein in normal human tears.
Curr Eye Res
; 34(10): 819-23, 2009 Oct.
Article
en En
| MEDLINE
| ID: mdl-19895309
ABSTRACT
PURPOSE:
To identify endogenous tear proteins immunoselected by antibodies from normal human ocular tears. MATERIALS ANDMETHODS:
Proteins were immunoselected from normal human tear samples without the addition of primary antibodies. Captured proteins were electrophoresed in polyacrylamide gels and silver stained. Human tears were also used as primary antibodies on immunoblots of tear proteins.RESULTS:
Lysozyme, lipocalin-1, and lactoferrin were immunoselected from normal human tear samples without the addition of primary antibodies. Antibodies from normal tears recognized lysozyme on immunoblots of tear proteins.CONCLUSION:
Normal human ocular tears contain antibodies to endogenous tear protein.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Autoanticuerpos
/
Lágrimas
/
Muramidasa
/
Lipocalina 1
/
Lactoferrina
Límite:
Female
/
Humans
Idioma:
En
Revista:
Curr Eye Res
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos