Surface modification with BSA blocking based on in situ synthesized gold nanoparticles in poly(dimethylsiloxane) microchip.
Colloids Surf B Biointerfaces
; 75(2): 608-11, 2010 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-19896345
ABSTRACT
A stable BSA blocking poly(dimethylsiloxane) (PDMS) microchannel was prepared based on in situ synthesized PDMS-gold nanoparticles composite films. The modified microchip could successfully suppress protein adsorption. The assembly was followed by contact angle, charge-coupled device (CCD) imaging, electroosmotic flow (EOF) measurements and electrophoretic separation methods. Contact angle measurements revealed the coated surface was hydrophilic, water contact angle for coated chips was 45.2 degrees compared to a water contact angle for native PDMS chips of 88.5 degrees. The coated microchips exhibited reproducible and stable EOF behavior. With FITC-labeled myoglobin incubation in the coated channel, no fluorescence was observed with CCD image, and the protein exhibited good electrophoretic effect in the modified microchip.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Albúmina Sérica Bovina
/
Técnicas Analíticas Microfluídicas
/
Dimetilpolisiloxanos
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Nanopartículas del Metal
/
Oro
Límite:
Animals
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Asunto de la revista:
QUIMICA
Año:
2010
Tipo del documento:
Article