Structural elucidation of the repeat unit in highly branched acidic exopolysaccharides produced by nitrogen fixing Burkholderia.
Glycobiology
; 20(3): 338-47, 2010 Mar.
Article
en En
| MEDLINE
| ID: mdl-19933228
ABSTRACT
Burkholderia kururiensis, strain M130, an endophytic diazotrophic bacterium isolated from rice roots, produces acetylated acidic exopolysaccharides which can be separated by anion exchange chromatography. These were characterized by nuclear magnetic resonance spectroscopy, methylation analysis and Smith degradation. The exopolysaccharides eluted with 0.5 M NaCl were produced when the bacterium was grown in a medium containing mannitol as the sole carbon source, and showed to be a mixture of two different polymers, composed of hepta or octasaccharide repeat units, consistent with following structure [structure see text]. The ability of diazotrophic Burkholderia to produce two exopolysaccharides that differ by the presence of a terminal glucosyl residue provides insight into polysaccharide function with potentially significant biological consequences in the endophytic-host plant interaction.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polisacáridos Bacterianos
/
Burkholderia
/
Fijación del Nitrógeno
Idioma:
En
Revista:
Glycobiology
Asunto de la revista:
BIOQUIMICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Brasil