Antimicrobial and immunomodulatory activities of an ovine proline/arginine-rich cathelicidin.
Int J Antimicrob Agents
; 35(3): 288-91, 2010 Mar.
Article
en En
| MEDLINE
| ID: mdl-20031378
ABSTRACT
In in vitro co-culture experiments, the ovine-derived cathelicidin OaBac5mini showed antimicrobial activity against Escherichia coli cells and modulated production of a cytokine by a mammalian inflammatory cell type (macrophage). Using atomic force microscopy, the morphology of peptide-treated E. coli bacteria showed no cell lysis, indicating an intracellular mode of action of the peptide leading to bacterial cell inhibition. At a concentration of 50microg/mL OaBac5mini, the peptide suppressed production of the inflammatory cytokine interleukin-12 by murine J774A cells that had been stimulated with Staphylococcus aureus strain Cowan; levels of other cytokines were unaffected. Thus, certain cationic peptides can enter and disrupt invading Gram-negative pathogens and may be able to modulate inflammatory responses induced by Gram-positive bacterial products.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ovinos
/
Péptidos Catiónicos Antimicrobianos
/
Factores Inmunológicos
/
Antibacterianos
Límite:
Animals
Idioma:
En
Revista:
Int J Antimicrob Agents
Año:
2010
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS