[Determination of scandoside methyl ester in Hedyotis chrysotricha by HPLC].

ABSTRACT

OBJECTIVE: To develop an HPLC method for determination of scandoside methyl ester in Hedyotis chrysotricha. METHOD: The determination was carried out on an HC-C18 column elnted with acetonitrile-water (793) as mobile phase, and the detection wavelength was set at 238 nm. RESULT: There is a good linearity in the range 22.08-552 mg L(-1) of scandoside methyl ester and the average recovery is 97.7% (n = 6), RSD 0.72%. CONCLUSION: This method is convenient, quick, and is applicable to the quality control of H. chrysotricha.