[Adenylate kinase and creatine kinase activity of rabbit blood serum following application of a tourniquet to the thigh]. / Adenilatkinaznaia i kreatinkinaznaia aktivnost' syvorotki krovi krolikov posle nalozheniia zhguta na bedro.
Vopr Med Khim
; (6): 728-32, 1977.
Article
en Ru
| MEDLINE
| ID: mdl-202085
A colorimetric method is described for estimation of the adenilate kinase activity in blood serum; the method is based on coupling of adenilate- and creatine kinase reactions and on estimation of the amount of creatine formed. Adenilate kinase activity in blood serum, estimated by the method, was shown to increase 4-fold after removing of tourniquet with subsequent normalization within the next day. The same data were obtained using a spectrophotometric method for estimation of adenilate kinase based on NADP reduction in coupled reactions with hexokinase and glucose-6-phosphate dehydrogenase. An increase in creatine kinase activity in blood serum occurred later on after removing of the tourniquet; it was more distinct (8.5-fold) and maintained longer as compared with the increase in adenilate kinase activity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfotransferasas
/
Adenilato Quinasa
/
Creatina Quinasa
/
Enfermedades Musculares
Límite:
Animals
Idioma:
Ru
Revista:
Vopr Med Khim
Año:
1977
Tipo del documento:
Article
Pais de publicación:
Rusia