[Preparation of polyclonal antibody against human Fkbp19].

AIM: To prepare the polyclonal antibody against human Fkbp19 for future functional studies of Fkbp19. METHODS: Prokaryotic expression vector pET21a-Fkbp19 was transformed into E.coli BL21-DE3, and then induced by IPTG. The recombinant protein was purified by Ni-NTA resin and used to immunize rabbits. Antiserums were used for Western blot and immunofluorescence to detect Fkbp19 in breast tumor cell. RESULTS: His-Fkbp19 fusion protein was successfully expressed in E.coli. High titer polyclonal antiserum was obtained by immunizing rabbits with purified His-Fkbp19 fusion protein. The antiserum could detect endogenous Fkbp19 protein in breast tumor cell very well by Western blot. CONCLUSION: The polyclonal antiserum against Fkbp19 was successfully generated.