Olfactory discrimination training up-regulates and reorganizes expression of microRNAs in adult mouse hippocampus.
ASN Neuro
; 2(1): e00028, 2010 Feb 26.
Article
en En
| MEDLINE
| ID: mdl-20309390
ABSTRACT
Adult male mice (strain C57Bl/6J) were trained to execute nose-poke responses for water reinforcement; then they were randomly assigned to either of two groups olfactory discrimination training (exposed to two odours with reward contingent upon correctly responding to one odour) or pseudo-training (exposed to two odours with reward not contingent upon response). These were run in yoked fashion and killed when the discrimination-trained mouse reached a learning criterion of 70% correct responses in 20 trials, occurring after three sessions (a total of approximately 40 min of training). The hippocampus was dissected bilaterally from each mouse (N = 7 in each group) and profiling of 585 miRNAs (microRNAs) was carried out using multiplex RT-PCR (reverse transcription-PCR) plates. A significant global up-regulation of miRNA expression was observed in the discrimination training versus pseudo-training comparison; when tested individually, 29 miRNAs achieved significance at P = 0.05. miR-10a showed a 2.7-fold increase with training, and is predicted to target several learning-related mRNAs including BDNF (brain-derived neurotrophic factor), CAMK2b (calcium/calmodulin-dependent protein kinase IIß), CREB1 (cAMP-response-element-binding protein 1) and ELAVL2 [ELAV (embryonic lethal, abnormal vision, Drosophila)-like; Hu B]. Analysis of miRNA pairwise correlations revealed the existence of several miRNA co-expression modules that were specific to the training group. These in vivo results indicate that significant, dynamic and co-ordinated changes in miRNA expression accompany early stages of learning.
Palabras clave
BDNF, brain-derived neurotrophic factor; Ct, threshold cycle value; DHPG, (S)-3,5-dihydroxyphenylglycine; LTP, long-term potentiation; MEF2, myocyte enhancer factor-2; NMDA, N-methyl-d-aspartate; RISC, RNA-induced silencing complex; RT-PCR, reverse transcription-PCR; TLDA, TaqMan® Low Density Arrays; TOP, terminal oligopyrimidine; dicer; learning; miRNA, microRNA; microRNA; olfactory discrimination; pre-miR, miRNA small hairpin precursor; pri-miR, primary miRNA gene transcript; snoRNA, small nucleolar RNA; synaptic plasticity
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Olfato
/
MicroARNs
/
Discriminación en Psicología
/
Hipocampo
/
Aprendizaje
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
ASN Neuro
Asunto de la revista:
NEUROLOGIA
/
QUIMICA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos