prp4 from Schizosaccharomyces pombe, a mutant deficient in pre-mRNA splicing isolated using genes containing artificial introns.
Mol Gen Genet
; 226(1-2): 305-9, 1991 Apr.
Article
en En
| MEDLINE
| ID: mdl-2034223
ABSTRACT
We have generated a bank of temperature-sensitive (ts) Schizosaccharomyces pombe mutant strains. About 150 of these mutants were transformed with a ura4 gene containing an artificial intron. We screened these ts mutants for mutants deficient in splicing of the ura4 intron. With this approach three mutants were isolated which have a general defect in the splicing process. Two of these mutants fall into the prp1 complementation group and one defines a new complementation group, prp4.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Schizosaccharomyces
/
ARN de Hongos
/
Precursores del ARN
/
Intrones
/
Empalme del ARN
/
Mutación
Idioma:
En
Revista:
Mol Gen Genet
Año:
1991
Tipo del documento:
Article