[Characteristics of CDR3 of TCR beta on CD8+ T cells in chronic hepatitis B patients].
Zhonghua Gan Zang Bing Za Zhi
; 18(3): 184-8, 2010 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-20380794
ABSTRACT
OBJECTIVE:
To analyze the characteristics of CDR3 of TCRbeta on CD8+ T cells in chronic hepatitis B patients.METHODS:
Eight patients with chronic hepatitis B (ALT more than 2 ULN) were enrolled in this study. CD8+ T cells were isolated from peripheral blood. RT-PCR was proformed to amplify the CDR3 of TCRbeta, and the PCR products were sequenced and analyzed.RESULTS:
The chronic hepatitis B patients showed obvious clonal expansion of T cell, and three perturbation patterns of T cell expansion were showed in the CDR3 of TCRbeta, including monoclonicity, oligoclonicity and skewed peak patterns. The number of perturbation families of CD8+ subpopulation was significantly higher than that of CD8- subpopulation (10.6+/-4.7 vs. 4.1+/-3.1, t = 6.619, P less than 0.01). In 3 out of 8 patients, the number of perturbation families of CD8+ subpopulation was also higher than that of PBMCs without depleting CD8+ subpopulation.CONCLUSIONS:
The characteristics of CDR3 of TCRbeta may help to understand the inflammatory response in CHB patients.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Receptores de Antígenos de Linfocitos T
/
Linfocitos T CD8-positivos
/
Hepatitis B Crónica
/
Regiones Determinantes de Complementariedad
Límite:
Adult
/
Humans
/
Male
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
China