[Overexpression of adiponectin prevents hepatocyte steatosis].
Zhonghua Gan Zang Bing Za Zhi
; 18(3): 213-6, 2010 Mar.
Article
en Zh
| MEDLINE
| ID: mdl-20380800
ABSTRACT
OBJECTIVE:
To investigate the effect of adiponectin on hepatocyte steatosis.METHODS:
L02 cells were transfected with pEGFP-N1-AdipoQ, a plasmid encoding pEGFP-adiponectin fusion protein, or pEGFP-N1. Lipid droplets in the hepatocytes were observed by oil red staining at 72 h. The contents of TG, FFA and glycerol in hepatocytes were measured.RESULTS:
Compared to cells transfected with pEGFP-N1-AdipoQ plasmid, much more lipid droplets were observed in cells transfected with pEGFP-N1 plasmid. TG, FFA and glycerol contents in L02 cells and L02/pEGFP-N1 cells were significantly higher than those in L02/pEGFP-N1-AdipoQ cells.CONCLUSIONS:
Overexpression of adiponectin prevent hepatocyte steatosis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Recombinantes de Fusión
/
Hepatocitos
/
Adiponectina
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Gan Zang Bing Za Zhi
Asunto de la revista:
GASTROENTEROLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
China