Formation of testicular structure under skin after auto-transplantation of dispersed testicular cells in the chicken.
Anim Reprod Sci
; 120(1-4): 125-8, 2010 Jul.
Article
en En
| MEDLINE
| ID: mdl-20392576
ABSTRACT
Transplantation of immature testis tissue under the skin provides a simple approach for the establishment of donor-derived-only spermatogenesis in birds. There are reports on the reconstruction of testicular structures from dissociated testicular cells in mammalian species. The present study aimed to evaluate whether, in birds, the transplantation of testicular cells under the skin could form a new testis and lead to fertility. Testicular cells from newly hatched chicks were auto-transplanted under the neck skin as a cell suspension or cell pellet. Testicular tissue was auto-transplanted under the neck skin as a control study. At the age of 1 year, both suspension and pellets of testicular cells formed testicular tissue under the skin. Only 11.3% of seminiferous tubules contained primary or normal lumens with normal spermatogenesis in testicular cell transplants while 80% of seminiferous tubules contained normal lumens in the testis tissue transplant. This technique, if further developed, would provide a novel system to manipulate and study spermatogenesis in birds.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Testículo
/
Trasplante Heterotópico
/
Pollos
Tipo de estudio:
Evaluation_studies
Límite:
Animals
Idioma:
En
Revista:
Anim Reprod Sci
Año:
2010
Tipo del documento:
Article
País de afiliación:
Canadá
Pais de publicación:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
/
PAISES BAJOS
/
THE NETHERLANDS